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MiSeq FGx System

Video demonstration of the MiSeq FGx Forensic Genomics System

View a recorded video of an exclusive live demonstration of the MiSeq FGx Forensic Genomics System, the first fully validated next-generation sequencing (NGS) solution. Designed exclusively for forensic genomics, the MiSeq FGx System is made up of the MiSeq FGx Instrument, the ForenSeqTM DNA Signature Prep Kit, and ForenSeq Universal Analysis Software. In this two-part webcast series, we’ll walk through the complete NGS workflow, from sample setup to data analysis.

Experience firsthand how this simple workflow maximizes information recovered from casework and database samples, as Brian Steffy, Senior Laboratory Manager in Applications and Communications Training, and Dr Steven Lee, Senior Technical Manager in Forensic Genomics at Illumina, walk you through.

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In the first episode of the series, Brian and Steven demonstrate the 5 simple steps to create sequencing-ready libraries. The protocol is covered and helpful insights are provided for ensuring high quality downstream results. By the end of this episode, you will know how to:

  • Achieve high-resolution and exceptional accuracy from 1 ng or less gDNA input —even with complex mixtures or degraded DNA
  • Perform sample multiplexing and automated processing
  • Access >200 genetic markers in a single test, including STRs currently utilized around the world, plus investigative leads, if desired

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This episode will focus on easy setup of the MiSeq FGx Instrument to perform sequencing, and how to analyze data, and generate reports with the ForenSeq Universal Analysis Software.

Starting with the pooled ForenSeq libraries from episode 1, Brian and Steven will guide you through run setup and sequencing using the intuitive user interface on the MiSeq FGx Instrument, followed by streamlined and powerful data analysis, and report generation using the ForenSeq Universal Analysis Software. At the conclusion of this episode, you will understand:

  • Reagent “load and go” using push-button operation
  • Real-time run monitoring using various forensic metrics and color coding to view performance and report status
  • How to go from sample to highly informative results and detail-rich reports—with no need for bioinformatics expertise

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Episode 1 & 2 Questions and Answers

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