If you're spending too much time and money identifying variants through Sanger sequencing or qPCR, download our guide to learn how targeted NGS can help you determine variants in less time and for less money.
Within a 2-week period, we get 24 700 kb data files that have been annotated and categorized on rareness and pathogenicity. 24 people every 2 weeks. It is just impossible to compare to a Sanger 1000-base-pair-at-a-time sequencing process.
– John Robinson, Ph.D at the Robarts Research Institute
*For $150 USD per sample, targeted resequencing can produce nearly 12x more data and cover hundreds more targets than Sanger sequencing. Calculations assume an NGS targeted run using the TruSeq® Custom Amplicon Low Input Library Prep Kit, MiSeq® v2 Sequencing Reagent Kit, a read length of 450bp, 700 targets, and 50x Coverage. Sanger sequencing assumptions include a read length of 800bp, 30 targets and a price per target (ie, price per rxn) of $5 USD.